By Richard J. Block, Emmett L. Durrum, Gunter Zweig
A guide of Paper Chromatography and Paper Electrophoresis presents a complete dialogue of the ideas of paper chromatography and paper electrophoresis. The booklet is geared up into components. half I on paper chromatography offers a effectively available resource for a number of the many makes use of and diversifications of paper chromatography. An attempt has been made to put in writing a pragmatic guide within which attempted and proved strategies, utilizing really basic apparatus and to be had reagents, are summarized. half II on paper electrophoresis discusses simple rules and technique. The emphasis all through has been at the separation of protein combinations, fairly blood serum. This displays the truth that it really is during this specific software that paper electrophoresis has up to now no longer been challenged by way of paper chromatography, while a few of the smaller molecules might be resolved both good or greater by means of the to this point extra broadly hired chromatographic tactics.
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Additional info for A Manual of Paper Chromatography and Paper Electrophoresis
Tubing is now attached, and a 5-mm. hole is blown in the bulb, F. , is prepared from 2-mm. capillary tubing and sealed to F as shown. Finally a control hole, H, 5 mm. in diameter, is blown in the top of the bulge; the outer tube is cut off and polished to be flush with the inner tube. The atomizer is attached by fixing the fluid feed tube in a two-hole rubber stopper so that the tube reaches nearly to the bottom of a 250-ml. Erlenmeyer flask. GENERAL METHODS 39 The De Vilbiss atomizer No. , Toledo, Ohio) is entirely satisfactory.
The a-c line current (115 volts) is passed through a voltage regulator, adjusted by a coarse and fine variable resistance, and is reduced to 6 volts by a stepdown transformer. The line is then connected to the light source, an automobile headlight, whose beam is adjusted by a focusing device to pass through the circular opening. ) Commercial photoelectric transmission densitometers are manufactured by W. M. Welch Mfg. , New York City, and other firms (see Figs. 15A, and 15B). Any electric recorder that has full-scale excursion for 200 microamperes or less and an input resistance of not more than 2500 ohms can be attached to the densitometer.
3, S. & S. 595, W. 4, W. 1 W. 3, S. &. S. 595, W. 1, W. 4 S. & S. 589 Black Ribbon, 595, 598, W. 3 W. 3, 1, 2, S. & S. 602 S. & S. 589, Blue, Red, White Ribbon, W. —Whatman filter paper; S. —Schleicher and Schüll filter paper; E. —Eaton and Dikeman filter paper. ^4. SOLVENTS (SEE TABLE II) As has been discussed previously, most developing solvents in partition chromatography are two-phase systems or mixtures of miscible organic solvents. The chemical nature of the solutes to be chromatographed usually suggests the class of solvents or mixtures to be used.
A Manual of Paper Chromatography and Paper Electrophoresis by Richard J. Block, Emmett L. Durrum, Gunter Zweig